Learnaboutgmp Community

Endotoxin in cleaning validation

Any help on how to validate the removal of endotoxin from product contact areas of equipment as part of a cleaning validation. I would think spiking endotoxin and introducing it into equipment used for aseptic processing a high risk approach. However simulation in a lab using coupons and rinsing techniques may be deemed inadequate. (Also, CIPs very difficult to simulate in lab) Any advice offered will be greatly appreciated.